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Habio α-galactosidase Introduction
Habio α-galactosidase is an enzyme used to hydrolyze or break α-1, 6-glycosidic bonds into galactosyl oligosaccharides (α-galactosides), liberating simpler, more usable sugars and eliminating its anti-nutrient effect. Thus, it improves the utilization of the energy and proteins in feedstuff.
Definition of Activity
One unit (U) of α-galactosidase is defined as the quantity of enzyme, which liberates 1μmol P-nitrophenol in one min at 37℃ and pH5.0.
Mechanisms
α-galactoside (such as raffinose and stachyose) is an anti-nutrient factor presenting in lots of plant seeds, especially the bean seeds have much high concentration. Poultry has no endogenous enzymes to digest the α-galactosides while swine can partially ferment it with the aid of intestinal microorganisms, but the CO2 and NH3 will be released, which would cause flatulence, nausea, borborgymus and diarrhea of animal. The α-galactosidase can degrade the α-galactoside into galactosyl oligosaccharides, which can not only eliminate the anti-nutrient effect but also provide energy for the animals.
Content of α-galactoside in common plant feed stuff
Plant seed | Raffinose(%) | Stachyose(%) | Total |
Soy bean | 1.21 | 4.22 | 5.43 |
Cottonseed | 6.91 | 2.36 | 9.27 |
Peanut | 0.33 | 0.99 | 1.32 |
Sunflower seed | 3.09 | 0.14 | 3.23 |
Wheat | 0.70 | 0.70 | |
Barley | 0.71 | 0.71 | |
Corn | 0.26 | 0.26 |
α-galactosidase can digest α-galactosides such as raffinose and stachyose. It can also synergize with β-mannanase to hydrolyze galactomannans contained in bean gums.
Graph4 Habio α-galactosidase Stability Curve
Table 2 The effect of metal ion on the enzyme activity of alpha-galactosidase
Metal Ion | Concentration/mol.L-1 | Relative enzyme activity/% | comment |
Fe2+ Ba2+ Mg2+ Ca2+ Mn2+ Cu2+ Zn2+ Control |
10-3 10-3 10-3 10-3 10-3 10-3 10-3 / |
97.31 89.37 117.24 120.18 82.46 96.06 95.43 100.00 |
0 - + + - 0 0 0 |
Note: + stands for positive effect, - stands for negative effect; 0 stands for unobvious effect( within 10%)
Product Function
Hydrolizing α-galactoside’s bonds, improving feed quality and allowing a better use of its carbohydrates and proteins, thus reducing cost.
Increasing feed intake of livestock, improving their growth performance, reducing the nitrogen output, thus creating less environmental pollution.
Specifications and Usage
Specification | Enzyme Activity | Usage |
Powder | 500-2,000U/g | Recommend using alpha-galactosidase in conjunction with mannanase. |
Granule | ||
Liquid | 500-2,000U/mL |
Package and Storage
Solid products: 25kg/Plastic woven bag.
Liquid products: 30kg/Plastic barrel.
Store in original package under sealed, room temperature condition for 12 months to solid form, and 6 months to liquid form (less than 25 ℃).
Avoid to be exposed to the sun, rain, high temperature and high humidity.
Technical Service
Offer α-galactosidase usage formula, enzyme activity test and related product technical service to ensure the using result.