Habio Acid Cellulase Animal Feed Additives High Thermostability

Cellulase can Break The Cell Wall, Releasing The Nutrients, Improving The Nutrients Digestibility

Product Introduction

Habio cellulase was produced by Habio using modern genetic engineering technology, microbial fermentation technology, and advanced post-processing techniques. It is composed of multiple ingredients and can degrade cellulose fibers to simple sugars efficiently, thus improving the utilization of raw ingredients, increasing the amount of unconventional materials, and reducing the cost.

Definition of Activity

One unit of cellulase is defined as the quantity of enzyme, which liberates 1μmol of reducing sugar from 4.0mmol/L sodium carbixymethyl cellulase substrate at 37℃ and pH5.5 in 1min.

Mechanism

Cellulase is a high-polysaccharides made of glucopyranose by β-1,4-glycosidic bond. Cellulase system consists of three major components: Endoglucanases (EG), cellobiohydrolases (CBH,), and β-glucosidases ( βG). EG acts on insoluble cellulose surface, breaks internal bonds to disrupt the crystalline structure of cellulase and expose individual cellulase polysaccharide chains, and makes cellulose chains hydration easily. CBH cleaves 2-4 units from the ends of the exposed cellulose chains produced by EG. βG hydrolyses the CBH product into individual monosaccharides. Through the synergistic action of above enzyme system, cellulose can be efficiently hydrolyzed to glucose.

Product Characteristics

1. It is produced by many second generation trichoderma reeseis that is transformed by Biological engineering technology, and it has a more reasonable enzyme system distribution, so it has more superior characteristic and stronger digestion efficiency compared with other cellulose.
2. Optimal genetic engineering strain, advanced production technology of submerged fermentation and unique post-processing ensure Habio cellulase high enzyme activity and good stability.
3. The optimal temperature is 60℃.
4. The optimal pH is 3.5-6.5;
5. Thermostable Treatment: Water bath for 5 min under different temperature, then test the residual enzyme activity.
6. pH Tolerance Treatment: Treatment for 90min under different pH, then test the residual enzyme activity.
7. Trypsin and Pepsin resistance:

Keep staying at Trypsin-Pepsin solution for 5.5 hours, the enzyme retention rate is 84.31%.

8. Good storage stability, convenient for transportation and storage.
9. Little affected by the metal ions and protease.

 Table1. The Effect of Metal Ion on the activity of Habio Cellulase

Note: + refers to activation; - refers to inhibition; 0 refers to no obvious impact (the effect on enzyme activity is within 10%)

Product Functions

1. Fully degradation of cellulose as oligosaccharides, reducing viscosity of chime;
2. Balancing the intestinal flora, improving animal immunity, reducing the diarrhea rate;

3. Breaking the cell wall, releasing the nutrients, improving the nutrients digestibility ;

4. Increasing the usage of unconventional raw materials, improving animal production performance, increasing customer benefits.

Specification

Usages

Package and Storage

Solid products: 25kg/Plastic woven bag.

Liquid products: 30kg/Plastic barrel.

Store in original package under sealed, room temperature condition for 12 months to solid form, and 6 months to liquid form (less than 25 ℃).

Avoid to be exposed to the sun, rain, high temperature and high humidity.

Technical Service

Offer Cellulase usage formula, enzyme activity test and related product technical service to ensure the using result.